Fig. 6
From: Early alterations of neurovascular unit in the retina in mouse models of tauopathy
TOMA treatment alleviates retinopathies. TOMA or control IgG was intravenously injected to P301L mice at 1 month of age and 1 week before sample collection at 3 months of age. a Retinal permeability. n = 7–8/group. b Leukostasis. Bar graph represents the number of stationary leukocytes adherent to the retinal vasculature per retina. n = 8–9/group. c Microglia were stained with anti-Iba1 antibody (purple) and images were taken at the NFL-GCL by confocal microscopy. Squares in the upper panel of images are zoomed in to show microglial activation. Bar graph represents the number of microglia at the NFL-GCL. n = 6/group. d, e Representative images of retinal flatmounts labeled with Tuj1 antibody (green) for RGCs and Opsin 4 antibody (purple) for ipRGCs at the peripheral retina. Arrowheads indicate the soma of ipRGCs. Bar graphs represent the numbers of RGCs (n = 9–10/group) and ipRGCs (n = 6/group) per field; eight images were taken at the peripheral retina for each sample and calculated as average value. Scale bar: 50 µm. * p < 0.05; ***p < 0.001; ****p < 0.0001 versus IgG