Fig. 6

Altered dlp mRNA levels modify TDP-43 induced locomotion defects. a Larval turning times for dlp mRNA overexpression in the w¹¹¹⁸ genetic background (control for OE experiments), TDP-43^WT, TDP-43^G298S and TBPH^RNAi. N = 29 for w¹¹¹⁸, 29 for dlp^OE, 30 for TDP-43^WT, 37 for TDP-43^WT dlp^OE, 31 for TDP-43^G298S, 36 for TDP-43^G298S dlp^OE, 32 for TBPH^RNAI, 31 for TBPH^RNAi dlp^OE. b Larval turning times for dlp RNAi in the attp40 genetic background (control for RNAi experiments), TDP-43^WT, and TDP-43^G298S. N = 30 for attp40, 31 for dlp^RNAi, 29 for attp40 TDP-43^WT, 37 for TDP-43^WT dlp^RNAi, 29 for attp40 TDP-43^G298S, 38 for TDP-43^G298S dlp^RNAi, 30 for TBPH^RNAi, 28 for TBPH^RNAi dlp^RNAi. Significance determined using the MannU Whitney Test or Holm Sidak’s multiple comparison test. *P_value < 0.05, **P_value < 0.01, ***P_value < 0.001. c–n Representative images of dlp^OE NMJs in the context of w¹¹¹⁸, TDP-43^WT, TDP-43^G298S and TBPH^RNAi stained for Dlp and HRP. o Quantification of Dlp intensity in NMJ terminal boutons. N = 5 for w¹¹¹⁸ dlp^OE, 5 for TDP-43^WT dlp^OE, 5 for TDP-43^G298S dlp^OE, 6 for TBPH^RNAi dlp^OE. Genotypes and stainings, as indicated. Scale bars: c 10 μm. *P_value < 0.05, **P_value < 0.01, ***P_value < 0.001. Error bars represent SEM