Fig. 3

RN2N IgG2a treated mice demonstrate an increased disinhibition-like behaviour following passive immunization of pR5 mice. a RN2N IgG isotype does not affect brain delivery in vivo following intravenous administration of Alexa-Fluor 647-conjugated RN2N. Representative images of Alexa Fluor 647-conjugated RN2N administered pR5 mice at 60 min post-treatment using a Bruker In Vivo MS FX Pro optical imaging system and quantification showing the mean fluorescence counts within the outlined region of interest were reported as logarithmic scale following the subtraction of the mean of control (− IgG) (n = 3 for each group). b Schematic of passive immunization treatment and behavioural analysis schedule in pR5 mice. c Elevated plus maze (EPM) results showing that mice treated with RN2N IgG2a spend more time in the open arms compared to the control and RN2N IgG1 treated mice. Schematic of EPM arena with its open and closed arms. Mean positional heat map within the EPM for each treatment group and quantification of % time spent in open arms over the duration of the test (n = 10 for each group). d Percentage (%) weight gain of treatment groups from start of treatment compared to end of treatment (n = 10 for each group). e–g Activity monitor (open field) showing no differences in treatment groups in e time spent in the centre zone, f resting time and g ambulatory time (n = 10 for each group). *p < 0.05, **p < 0.01