Fig. 2

a RN2N IgG2a treatment demonstrates enhanced BV2 microglial cell phagocytosis of tau and increased transcription of pro-inflammatory cytokines. Flow cytometric analysis and quantification of tau-pHrodo fluorescence in BV2 microglial cells treated with either RN2N IgG1, RN2N IgG2a, control IgG1 or control IgG2a (***p < 0.001, ****p < 0.0001; n = 3 for each group). b Representative microscopy images of BV2 microglial cells treated with RN2N and human recombinant tau showing phagocytosis of RN2N IgG (green) and internalized Tau-pHrodo (red) complexes. Nuclei were stained with DAPI in blue. Scale bar = 20 μm. c BV2 microglial cells were treated with recombinant human tau with or without RN2N IgG, and TNFα and IL1-β transcription was analysed using sqRT-PCR at 2 or 8 h post-treatment (*p < 0.05; n = 3 for each group)