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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Tau antibody isotype induces differential effects following passive immunisation of tau transgenic mice

Fig. 1

Generation and characterisation of RN2N IgG1 and IgG2a isotypes. a Schematic of generation of RN2N IgG1 and IgG2a monoclonal antibodies using mAbXpress IgG vectors. b Single-cycle kinetics sensorgram of RN2N IgG1 to full-length human tau and summary table of RN2N IgG1 and RN2N IgG2a binding affinity (KD) to full-length human tau determined using surface plasmon resonance. The KD of RN2N IgG1 and RN2N IgG2a was 407 nM and 381 nM, respectively. c Western blot of mouse soluble brain extracts probed with RN2N IgG1 and IgG2a antibodies indicating similar binding profiles to tau (pR5 = pR5 tau transgenic mouse; WT = wild-type mouse; KO = tau knock-out mouse)

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