Fig. 6

Element maps in human SN nigrosome 1 in controls (Co) and patients with PD, obtained by µPIXE. Brightfield microscope images (top row) show the immunocytochemical reaction for cell-type specific antigens providing the basis for definition of regions of interest for the subsequent PIXE imaging (below). The ultrapure nickel-enhancement of the immunoreaction allows to allocate the µPIXE element signals to specific cell types. The iron map was used to quantify the local concentrations of iron in different cell types (see Fig. 7). Scale bar top left 20 µm applies to all images