Fig. 2From: Cell specific quantitative iron mapping on brain slices by immuno-µPIXE in healthy elderly and Parkinson’s diseaseSelection of regions of interest for cellular iron quantification. In the nickel (Ni) map of the immunocytochemical preparation of Hu C/D, the region of interest (ROI) is defined by a spline (in green) marking the cell shape. This ROI applies also to the other element maps from which the concentrations can be extracted. HU C/D: neuronal marker, S: sulphur, Fe: iron. Scale bar 20 μm, applies to all imagesBack to article page