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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Cell specific quantitative iron mapping on brain slices by immuno-µPIXE in healthy elderly and Parkinson’s disease

Fig. 2

Selection of regions of interest for cellular iron quantification. In the nickel (Ni) map of the immunocytochemical preparation of Hu C/D, the region of interest (ROI) is defined by a spline (in green) marking the cell shape. This ROI applies also to the other element maps from which the concentrations can be extracted. HU C/D: neuronal marker, S: sulphur, Fe: iron. Scale bar 20 μm, applies to all images

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