Fig. 1From: Cell specific quantitative iron mapping on brain slices by immuno-µPIXE in healthy elderly and Parkinson’s diseaseSample preparation for proton beam analysis. The antibody (Ab)-complex, bound to a cell-type-specific antigen is enhanced with high-purity nickel (violet spheres). Since nickel is specifically identifiable in the PIXE map, it can be used as a marker for any antigen of interest. This combination of antigen labelling with subsequent nickel amplification allows for the application of quantitative PIXE analysis to any immunocytochemical preparationBack to article page