Fig. 7

Tau seeding does not conform to one-hit dynamics. a Levels of seeding in HEK293 cells expressing P301S tau-venus following titration of recombinant tau assemblies in the presence of Lipofectamine 2000 transfection reagent. Percent of cells seeded was determined by high content microscopy and expressed as percent maximum observed seeding. A one-hit curve was fitted using values outside the plateau. b Levels of seeded aggregation in P301S tau transgenic OHSCs treated with recombinant tau assemblies applied to the apical surface of OHSCs. Percent AT8 area was measured and expressed as percent of maximum seeding (raw data in Additional file 1: Supplementary Fig. 3). For illustration, a one-hit curve was extended from a data point below saturation. c Infection of P301S tau transgenic OHSCs with a titration of AAV1/2.hSyn-GFP, with a one-hit curve fitted using all data points (Slices from N = 3 mice per condition)