Fig. 6

Tau seeding does not occur at low dose. a OHSCs challenged with 30 nM, 100 nM and 300 nM of recombinant tau assemblies or with buffer only, supplied into the media beneath the insert in a volume of 1 ml. Scale bars, 50 µm. b Quantification of AT8 immunoreactivity in OHSCs treated as above. c Quantification of seeding levels in P301S OHSCs, upon the addition of recombinant tau assemblies or buffer only to the apical surface of individual slices. Statistical significance determined by Kruskal–Wallis Test by ranks and Dunn’s multiple comparisons test (Slices from N = 6 mice per condition. ****P < 0.0001)