Fig. 2

Characterisation of seed competent tau assemblies. a Time course of recombinant P301S tau assembly, monitored by Thioflavin T fluorescence. b Representative transmission electron microscopy image of heparin assembled P301S tau. c Aged P301S tau transgenic mouse brain homogenate was immunoblotted for human tau (HT7 antibody) to detect transgenic tau and for cyclophilin B which served as a loading control. Presence of sarkosyl insoluble (SI) tau was confirmed with the HT7 antibody and AT100 (tau phosphorylated at pT212, pT214). Lanes represent preparations from different mice which were subsequently pooled. d Representative images from HEK293 cells expressing P301S tau-venus 48 h after challenge with either recombinant P301S tau assemblies or mouse SI tau in the presence of LF2000. e, f Tau-venus aggregates observed following challenge with tau assemblies stain with AT8 and AT100 demonstrating that the induced tau aggregates are phosphorylated. Scale bars, 20 µm