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Fig. 8 | Acta Neuropathologica Communications

Fig. 8

From: Prolonged activation of cytomegalovirus early gene e1-promoter exclusively in neurons during infection of the developing cerebrum

Fig. 8

The activation of MCMV e1-pro-1373 exclusively in neurons, but not in differentiated CX astrocytes during the second postnatal week. Fluorescence views of EGFP (a, d), immunofluorescence for NeuN (b) or GFAP (e) and merged images of EGFP and each marker (c, f) in the CXs of neonatal mice infected with rMCMC1373 at 11dpi are shown. a Almost all EGFP+ cells demonstrate a morphology of the pyramidal neuron. b In the same field as a, NeuN (purple) is expressed in the nuclei of CX neurons. c A merged image of a and b. Nuclear areas of EGFP+-CX neurons appear whitish because of the co-localization of EGFP and NeuN, indicating that e1-pro-1373 is distinctly activated in neurons. d Another fluorescence image of EGFP. e In the same field as d, GFAP (red) is expressed in the cytoplasm of differentiated astrocytes, a morphology of which is distinctly different from that of EGFP+-pyramidal neurons in d. f A merged image of d and e. EGFP is not accurately co-localized with GFAP, indicating that the activation of e1-pro-1373 does not occur in differentiated CX astrocytes. Scale bars: af 50 µm

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