Fig. 4

Identification of EGFP⁺ cell types in primary neuronal cultures infected with rMCMV1373 (a–f) or rMCMV448 (g–l) as shown in Fig. 3i–t. Immunofluorescence were performed using antibodies against NeuN (a–c, g–i) and GFAP (d–f, j–l) as neuronal and glial cell makers, respectively. Nuclei are stained with DAPI. In each rMCMV-infected culture, fluorescence views of EGFP (a, d, g, j), immunofluorescence for NeuN (purple) (b, h) and GFAP (red) (e, k) and merged images of EGFP and cell markers (c, f, i, l) are shown. Arrow heads indicate EGFP⁺/NeuN⁻ or EGFP⁺/GFAP⁺ round cells. In rMCMV1373-infected primary neuronal cultures almost all EGFP⁺ cells are neurons (a–c), while in rMCMV448-infected cultures there are very few EGFP⁺ neurons (g–i). In both rMCMV1373- and rMCMV448-infected primary neuronal cultures (f, l), EGFP is not expressed GFAP⁺ differentiated astrocyte with a coral-like shape, though very few EGFP⁺/GFAP⁺ round cells are found. Scale bars: a–c, g–i 25 µm; d–f, j–l 50 µm