Fig. 2
Viral growth in MEFs (a), primary neuronal cultures (b) and neonatal mouse brains (c) infected with wild type MCMV (■), rMCMV1373 (●) or rMCMV448 (▲). MEF and primary neuronal cultures were infected with MCMVs at MOIs of 1 and 3, respectively. Neonatal mice were intracerebrally infected with 4 × 104 PFU of MCMVs at 1 day after birth. Viral loads in each culture supernatant or mouse brain were determined by infectious viral titers. The dotted line indicates the detection limit. Mean ± SEM of 3 samples in infected cultures or mouse brains in each experimental group are shown