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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Prolonged activation of cytomegalovirus early gene e1-promoter exclusively in neurons during infection of the developing cerebrum

Fig. 1

Constructions of rMCMV448 and rMCMV1373. The arrangement of the original MCMV-e1-pro (position nt 161605 to 162977, black and gray arrow), M112/113 (e1) gene (position nt 162978 to 165076, white arrow) and the insertion site of e1-pro-EGFP (right black box) in the wild type MCMV genome are shown. In this study, an e1-pro-EGFP cassette consisting of the e1-pro fragment (nt -1373 or -448 to + 38 relative to the transcription start site, black and gray or gray arrow, respectively) and EGFP gene with an SV40-derived polyadenylation signal (striped arrows) was inserted into the position between nt 184431 and 187158 (right black box) in wild type MCMV genome by homologous recombination. This recombination causes the deletion of the nt 2728 sequence including the greater part of the M128 gene (black line). During the infection of recombinant viruses, the activation of the inserted e1-pro can be in situ detected as the expression of EGFP. The detailed construction, preparation method and verification of rMCMVs are shown in Additional file 1: Fig. S1

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