Table 2 Overview of the primary antibodies used in the present study
Antibody | Fluorophore | Clone | Species | Dilution | Source | Product number | |
|---|---|---|---|---|---|---|---|
Tissue | In vitro | ||||||
IHQ | FC | ||||||
CD45 | BV425 | 30-F11 | Rat monoclonal | Â | 1:200 | BioLegend | 103,134 |
CD3É› | AF700 | 500-A2 | Armenian Hamster monoclonal | Â | 1:100 | BioLegend | 100,320 |
CD4 | BUV395 | GK1.5 | Rat monoclonal | Â | 1:250 | BD Biosciences | 565,974 |
CD8a | PerCP-Cy5.5 | 53–6.7 | Rat monoclonal |  | 1:200 | BioLegend | 100,733 |
CD11b | BV510 | M1/70 | Rat monoclonal | Â | 1:500 | BioLegend | 101,263 |
CD25 | PE | PC61 | Rat monoclonal | Â | 1:200 | BioLegend | 102,007 |
MHC-II | PerCP-710 | AF6-120.1 | Mouse monoclonal |  | 1:200 | eBioscience | 46–5320-80 |
TLR4 | PE-Cy7 | SA15-21 | Rat monoclonal | Â | 1:250 | BioLegend | 145,407 |
Ly6C | BV785 | HK1.4 | Rat monoclonal | Â | 1:2000 | BioLegend | 128,041 |
Ly6G | AF700 | 1A8 | Rat monoclonal | Â | 1:100 | BioLegend | 127,622 |
NeuN | Â | A60 | Mouse monoclonal | 1:1000 | Â | Millipore | MAB377 |
NeuN | Â | A60 | Rabbit polyclonal | 1:4000 | Â | Millipore | MAB377 |
DCX | Â | Â | Rabbit polyclonal | 1:1000 | Â | Abcam | Ab18723 |
BrdU | Â | BU1/75 | Rat monoclonal | 1:150 | Â | Abcam | Ab6326 |
Iba1 |  | NCNP24 | Rabbit polyclonal | 1:1000 |  | Wako | 019–19,741 |
CD3 | Â | SP7 | Rabbit polyclonal | 1:100 | Â | Abcam | ab16669 |
Secondary antibody | AF594 | Â | Donkey anti-rat | 1:500 | Â | Thermo Fisher | A-21209 |
Secondary antibody | AF647 | Â | Donkey anti-mouse | 1:500 | Â | Thermo Fisher | A-31571 |
Secondary antibody | AF488 | Â | Donkey anti-rabbit | 1:500 | Â | Thermo Fisher | A-21206 |
Secondary antibody |  |  | Biotinylated goat anti-rabbit | 1:1000 |  | Vector Laboratories | BA-1000–1.5 |