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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Gut microbial dysbiosis after traumatic brain injury modulates the immune response and impairs neurogenesis

Fig. 6

Long term alterations of local immunity after microbiota dysbiosis at the time of TBI. a Experimental design. Graphs depict b Richness index and c Shannon diversity index. Genera with a frequency > 3% annotated, mean values are plotted ± SEM, unpaired t-test, *** p < 0.001, n = 3 per group. d Gating strategy of CD3+ cells and CD25+ cells in the ipsilateral hippocampi. e–l Quantification of the cell absolute numbers in the brain. Mean values are plotted ± SEM, two-way ANOVA followed by Tukey's multiple comparisons. F statistic for antibiotic*injury presented unless otherwise noted (*p < 0.05, **p < 0.01 ***p < 0.001, ****p < 0.0001). n = 7–8 mice per group. e leukocytes (CD45), f CD3 T cells (CD11b−CD3+), Finjury (1,30) = 15.52, p = 0.0005; g CD4 T cells (CD11b−CD3+CD4+), F(1,30) = 5.704, p = 0.023; h CD8 T cells (CD11b−CD3+CD8+), i DN T cells (CD11b−CD3+CD4−CD8−), Finjury (1,30) = 18.91, p = 0.0001; j Treg cells (CD11b−CD4+CD25+), hippocampus, F(1,30) = 9.011, p = 0.0054, cortex, F(1,30) = 5.443, p = 0.0265; k monocytes (CD45highCD11b+Ly6C+), and l microglia (CD45lowCD11b+). m MFI of TLR4 in the microglia population, Finjury (1,11) = 19.85, p = 0.0010. Abbreviations: VNAM, vancomycin, neomycin-sulfate, ampicillin and metronidazole; CCI, controlled cortical impact. Hip: hippocampus; Amy, amygdala; Cx, cortex

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