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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: C11orf95-RELA fusion drives aberrant gene expression through the unique epigenetic regulation for ependymoma formation

Fig. 4

C11orf95 determines the RELAFUS1-binding region through the unique GC-rich motif. a Schematic of RCAS vector constructs (See Methods) b Subcellular localization of RELA-HA, C11orf95-HA and RELAFUS1-HA in DF-1 cells. c Subcellular localization of GFP and GFP-fusion proteins. DF-1 cells expressing the relevant RCAS virus were observed with fluorescent microscopy. Representative images were shown in b and c. d, f RCAS vectors expression in 293T/tv-a cells. e, g Relative Nanoluc reporter activity to the RELAFUS1-RE-luciferase (MEME-2) normalized to the Firefly luciferase activity and GFP cells in the 293T/tv-a cells expressing the indicated RCAS virus as shown in the d or f. (e, n = 6; g, n = 5 or 9 in technical triplicate) Analysis was done using paired two-tailed t-test (e) or RM one-way ANOVA (g). *p < 0.05

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