Fig. 6

Overexpression of α-synuclein reduced CHCHD2 expression in MN9D cells, possibly by altering the expression and subcellular localization of p300. a Reduced mRNA expression of CHCHD2 in MN9D cells after overexpression of α-synuclein (F(2, 6) = 35.31, one-way ANOVA, n = 3; p < 0.001 for Wt α-syn vs. Vector, p < 0.01 for A53T α-syn vs. Vector). b Reduced protein expression of CHCHD2 in MN9D cells after overexpression of α-synuclein [F(2, 6) = 20.75, one-way ANOVA, n = 3; p < 0.05 for Wt or A53T α-syn vs. Vector]. c Direct interaction between p300 and CHCHD2 promoter as revealed by ChIP result. Overexpression of both wild type and A53T α-synuclein reduced the interaction of p300 and CHCHD2 promoter [F(2, 6) = 73.44,one-way ANOVA, n = 3; both p < 0.001, compared to Vector]. Histone 3 (H3) was used as positive control. d Decreased mRNA expression of p300 after overexpression of α-synuclein [F(2, 6) = 130.2, one-way ANOVA, n = 3; both p < 0.001, compared to Vector]. e Protein expression of p300 [F(2, 6) = 41.95, one-way ANOVA, n = 3; both p < 0.001, compared to Vector]. f Reduced nuclear distribution of p300 after overexpression of α-synuclein revealed by immunofluorescence (F (2, 154) = 12.8, one-way ANOVA, p < 0.05 or 0.01 or 0.0001). g Reduced localization of p300 in nucleus revealed by western blot (p < 0.05, Mann–Whitney U test, n = 3); Increased localization of p300 in cytoplasm (p < 0.05, Mann–Whitney U test, n = 3)