Fig. 4

Reduced expression of CHCHD2 in the erythrocytes and substantia nigra of A53T α-synuclein transgenic mice. a Compared to non-transgenic wild type mice, normalized mRNA of CHCHD2 in erythrocytes of A53T α-synuclein transgenic mice was significantly decreased from 1.11 ± 0.20 to 0.27 ± 0.03 (p < 0.01, Mann–Whitney U test, n = 6). b Western blot results showed a significantly reduced protein expression of CHCHD2 in erythrocytes of transgenic mice (p < 0.01, Mann–Whitney U test, n = 3). Upper: representative Western blot images. β-actin was used as an internal control. Wt: non-transgenic wild type mice. Tg: A53T transgenic mice. Lower: quantification of Western blots. c Real-time qPCR also detected a reduced mRNA expression of CHCHD2 in substantia nigra of transgenic mice (p < 0.01, Mann–Whitney U test, n = 6). d CHCHD2 protein was decreased from 1.21 ± 0.21 in substantia nigra of wild type mice to 0.21 ± 0.10 in substantia nigra of transgenic mice (p < 0.05, Mann–Whitney U test, n = 3). Upper: representative Western blot images. β-tubulin was used as an internal control. Lower: quantification of Western blots. Of note, the sample number (N) is different because mRNA and proteins were measured in two different sets of animals