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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Reduced erythrocytic CHCHD2 mRNA is associated with brain pathology of Parkinson’s disease

Fig. 2

Reduced expression of CHCHD2 in post-mortem substantia nigra slices of PD patients. a Representative images of co-staining of CHCHD2 (green) with neuronal marker NeuN (blue) and dopaminergic neuronal marker TH (red), showing expression of CHCHD2 in neurons. Scale bar: 10 μm. b Representative images of co-staining of CHCHD2 (green) with astrocyte marker GFAP (red), showing expression of CHCHD2 in astrocytes. Scale bar: 10 μm. c Representative images of co-staining of CHCHD2 (green) with microglial marker Iba1 (red), showing expression of CHCHD2 in a minority of microglia. Scale bar: 10 μm. d Quantification of the percentage of CHCHD2 positive cells by cell type in Iba1, GFAP or NeuN positive cells. e and f Immunofluorescence staining images of substantia nigra of control and PD brain slices probed with the antibody against CHCHD2 (green) and TH (red). Scale bar = 20 μm. g Quantification of TH+ and CHCHD2+ cells in substantia nigra, demonstrating lower numbers of surviving neuronal cells and surviving CHCHD2+ cells in PD patients. p < 0.001, multiple t-test, n = 3 each. h Quantification of immunofluorescent signal demonstrating reduced CHCHD2 immunofluorescent intensity in substantia nigra (p < 0.001, Mann–Whitney U test, n = 3)

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