Two distinct conformers of PrPD type 1 of sporadic Creutzfeldt–Jakob disease with codon 129VV genotype faithfully propagate in vivo

Table 2 Histopathological and PrP immunohistochemical (IHC) features of inoculated Tg mice (1st passage)

Mouse line	Inoculum	H.E			PrP IHC pattern
Mouse line	Inoculum	SD topography	Vacuole size	Plaques	Cerebral Cortex (CC)	Cerebellum
Tg129V	VV1²⁰	↑CC- ↓Th^a	Medium	No	Granul. Aggreg	Focal, Grl. L
	VV1²¹⁻²⁰*	↑CC- ↓Th	Medium	No	Granul. Aggreg	Focal, Grl. L
	VV1²¹	↑CC- ↓Th	Medium	No	Granul. Aggreg	Negative
Tg129M	VV1²⁰	↑CC- ↓Th	Large	No	Granul. Aggreg	Focal, Grl. L
	VV1²¹⁻²⁰*	↑CC- ↓Th	Large	No	Negative	Negative
	VV1²¹	Negative
Tg129V	VV2	↓CC- ↑Th	Small	Yes, BS	Plaque-like	Negative
Tg129M	VV2	↓CC- ↑Th	Small	Yes, widespread	Plaques	Plaques & plaque-like

^aArrows depict gradients of lesion severity: upward arrow = maximum; downward arrow = minimum; H.E.: Hematoxylin–eosin; SD: spongiform degeneration; CC: cerebral cortex; Th: thalamus; Granul. Aggreg.: granule aggregate; Grl. L.: granule cell layer; BS: brainstem

ISSN: 2051-5960