Fig. 1From: Two distinct conformers of PrPD type 1 of sporadic Creutzfeldt–Jakob disease with codon 129VV genotype faithfully propagate in vivoCharacterization of mouse brain resPrPD. T1 and T2 and their superscripts atop of each blot refer to the mouse resPrPD type and variant; VV120, VV121 and VV2 refer to resPrPD obtained from sCJDVV cases. a, i-ii: The unglycosylated isoform of the mouse resPrPD migrated to either ~ 20 kDa (i, “20”) or ~ 21 kDa (ii, “21”) accurately replicating the resPrPD of VV120 and VV121, respectively; iii and v: VV121−20* inoculum was reproduced as T121 (iii, “21”) with the additional presence of a Tohoku-2-immunoreactive ~ 19 kDa band (v, “19”); iv: Tohoku-2 showing a negative (Neg.) immunoreactivity for T120 and T121 that were detected by 3F4 in i and ii, respectively. b, i and iii: Mice challenged with VV120 (i) and VV121−20* (iii) generated resPrPD T121−20 with a predominant ~ 20 kDa fragment (arrowhead, i) over a ~ 21 kDa band (arrow, i). ii: Mice challenged with VV121 were all negative; bracket: mouse immunoglobulins. iv and v: Tohoku-2 immunoreacted with T2 in mice challenged with VV121−20* (v) but not in those inoculated with VV120 or VV121 (iv). c-d: Magnification of unglycosylated resPrPD fragments. C: Mouse resPrPD migrated to ~ 21 kDa in Tg129V inoculated with VV121 (lane 1) or VV121−20* (lane 3). d: A small fragment of ~ 21 kDa migrating above a prominent one of ~ 20 kDa (lane 1), or a single band of ~ 20 kDa (lane 2), was detected in Tg129M or Tg129V inoculated with VV120. e: Mice challenged with VV2. Top panel, Ab 3F4: Mouse resPrPD migrated to either ~ 19 kDa (“19”) in Tg129V or ~ 20 kDa (“20”) in Tg129M. Bottom panel, Ab Tohoku-2: The mouse resPrPD of ~ 19 kDa, but not the ~ 20 kDa band, was detected by Tohoku-2Back to article page