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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Two distinct conformers of PrPD type 1 of sporadic Creutzfeldt–Jakob disease with codon 129VV genotype faithfully propagate in vivo

Fig. 1

Characterization of mouse brain resPrPD. T1 and T2 and their superscripts atop of each blot refer to the mouse resPrPD type and variant; VV120, VV121 and VV2 refer to resPrPD obtained from sCJDVV cases. a, i-ii: The unglycosylated isoform of the mouse resPrPD migrated to either ~ 20 kDa (i, “20”) or ~ 21 kDa (ii, “21”) accurately replicating the resPrPD of VV120 and VV121, respectively; iii and v: VV121−20* inoculum was reproduced as T121 (iii, “21”) with the additional presence of a Tohoku-2-immunoreactive ~ 19 kDa band (v, “19”); iv: Tohoku-2 showing a negative (Neg.) immunoreactivity for T120 and T121 that were detected by 3F4 in i and ii, respectively. b, i and iii: Mice challenged with VV120 (i) and VV121−20* (iii) generated resPrPD T121−20 with a predominant ~ 20 kDa fragment (arrowhead, i) over a ~ 21 kDa band (arrow, i). ii: Mice challenged with VV121 were all negative; bracket: mouse immunoglobulins. iv and v: Tohoku-2 immunoreacted with T2 in mice challenged with VV121−20* (v) but not in those inoculated with VV120 or VV121 (iv). c-d: Magnification of unglycosylated resPrPD fragments. C: Mouse resPrPD migrated to ~ 21 kDa in Tg129V inoculated with VV121 (lane 1) or VV121−20* (lane 3). d: A small fragment of ~ 21 kDa migrating above a prominent one of ~ 20 kDa (lane 1), or a single band of ~ 20 kDa (lane 2), was detected in Tg129M or Tg129V inoculated with VV120. e: Mice challenged with VV2. Top panel, Ab 3F4: Mouse resPrPD migrated to either ~ 19 kDa (“19”) in Tg129V or ~ 20 kDa (“20”) in Tg129M. Bottom panel, Ab Tohoku-2: The mouse resPrPD of  ~ 19 kDa, but not the ~ 20 kDa band, was detected by Tohoku-2

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