Fig. 2

Pathological α-Syn induce nociceptive impairment. a Schematic illustration of the experimental design. Three-month-old M83^+/− were bilaterally injected with vehicle (PBS, pH 7.4, n = 6), mouse monomeric α-Syn (2 × 5 μl at 2 mg/ml, n = 8) or mouse α-Syn PFF (2 × 5 μL at 2 mg/ml, n = 8), by intramuscular injection into the gastrocnemius muscle. Hindlimb clasping behaviour was monitored and scored regularly. At 45 dpi, mice were tested for hindlimb clasping, sensory and motor nerve conduction and plantar Von Frey filaments, before being euthanized. Tissues, including brain, spinal cord, dorsal roots and lumbar (L3–L5) dorsal root ganglia (DRG) were then snap frozen and stored at − 80 °C until use, or fixed and processed for immunohistochemistry. b Hindlimb clasping was scored on a scale from 0 to 3 as a function of dpi and displayed as mean ± SEM. The PBS- and monomeric α-Syn-injected mice did not develop clasping. Results shown as mean ± SEM, as determined by two-way ANOVA followed by Tukey’s multiple comparisons test. ****P < 0.0001. At 45 dpi mice were tested for c Motor nerve conduction velocity. Error bar indicate mean ± SEM as determined by one-way ANOVA followed by Tukey’s multiple comparison test. *P < 0.05. d Sensory nerve conduction velocity. Results shown as mean ± SEM as determined by one-way ANOVA followed by Tukey’s multiple comparison test. *P < 0.05. e Mechanical allodynia using the Von Frey test. Error bars indicate mean ± SEM as determined by one-way ANOVA followed by Tukey’s multiple comparison test. *P < 0.05