Table 1 Primary antibodies used for Western blotting
Antibody | Specificity | Source | Type | Dilution |
|---|---|---|---|---|
AT270 | Thr181 | ThermoFisher MN1050 | Mouse monoclonal | 1:600 |
Phospho-Tau (Thr181) | Thr181 | Cell Signaling 12885 | Rabbit monoclonal | 1:1200 |
Phospho-Tau (Thr181) | Thr181 | ThermoFisher 710561 | Rabbit oligoclonal | 1:1000 |
Phospho-Tau (Ser404) | Ser404 | ThermoFisher 44-758G | Rabbit polyclonal | 1:1000 |
PHF13 | Ser396 | Cell Signaling 9632 | Mouse monoclonal | 1:1000 |
Phospho-Tau (Thr231) | Thr231 | ThermoFisher 710126 | Rabbit oligoclonal | 1:1000 |
CP13 | Ser202 | Dr. Peter Davies | Mouse monoclonal | 1:500 |
AT8 | Ser202/Thr205 | ThermoFisher MN1020 | Mouse monoclonal | 1:600 |
Tau | Total tau | Proteintech 10274-1-AP | Rabbit polyclonal | 1:600 |