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Fig. 7 | Acta Neuropathologica Communications

Fig. 7

From: Alzheimer’s disease brain contains tau fractions with differential prion-like activities

Fig. 7

Heat treatment does not passivate the prion-like activity of O-tau. a-c Sup-tau and HS-tau were similar in phosphorylation and truncation. Sup-tau and HS-tau were analyzed by Western blots developed with polyclonal tau antibodies (a), monoclonal tau antibodies (b), and site-specific and phosphorylation-dependent antibodies (c). d, e Sup-tau, but not HS-tau, was able to capture tau. Various amount of Sup-tau and HS-tau were applied on NC membranes. One membrane was subjected to tau overlay assay by sequential incubation of HEK-293FT/HA-tau151-391 cell extract, anti-HA (d, left), and one membrane was subjected to analysis of tau level by dot-blot developed with a mixture of R134d and 92e (d, right). The captured tau (e, left) and the dotted tau levels (e, right) detected in panel d were plotted against applied Sup-tau and HS-tau. f, g Heat treatment did not affect O-tau to recruit tau. Various amounts of O-tau were applied onto NC membrane. One membrane was boiled, and the other membrane was treated with the same buffer at RT as a control. The membranes were incubated with cell extract containing HA-tau151-391. The captured tau (f, left) and applied tau (f, right) were analyzed as described above and plotted with the levels of O-tau (g). A.U., arbitrary units

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