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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Nucleolar stress in C9orf72 and sporadic ALS spinal motor neurons precedes TDP-43 mislocalization

Fig. 3

Nucleolar size reduction occurs in neurons with and without antisense RNA foci in C9-ALS. a Nucleolar area is identified by fibrillarin staining (green), nuclear area is identified by DAPI and cytoplasmic area is identified by autofluorescence in control, C9-ALS and SALS spinal motor neurons. C-terminal TDP-43 immunofluorescence (cyan) was always nuclear in control neurons and was either nuclear or mislocalized to the cytoplasm in ALS neurons. Antisense RNA foci are identified by FISH staining (red) (nucleolar antisense RNA foci are show in the insert, arrowheads). b–g Nucleolar area (b, c), nuclear area (d, e) and cytoplasmic area (f, g) are decreased in C9-ALS and SALS neurons with or without nucleolar antisense RNA foci compared to controls

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