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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Nucleolar stress in C9orf72 and sporadic ALS spinal motor neurons precedes TDP-43 mislocalization

Fig. 2

Gamma motor neurons are identified by ESRRG immunohistochemistry and immunofluorescence. a Immunohistochemistry of ESRRG in control, C9-ALS and SALS lumbar spinal cord with cresyl violet counterstain. Alpha motor neurons (third column) lack nuclear ESRRG immunofluorescence. Gamma motor neurons (fourth column) have nuclear ESRRG immunofluorescence. Magnification scales are consistent for each column. b Distribution of cytoplasmic area for gamma and alpha motor neurons in control, C9-ALS and SALS lumbar spinal cord (dashed line: threshold for alpha motor neurons). c–f Stratification of immunofluorescence data. c Distribution of cytoplasmic area from un-stratified immunofluorescence data. d Comparison of similar distribution of cytoplasmic area in sub-sample of control, C9-ALS and SALS alpha motor neurons (cytoplasmic area thresholds at 2000 µm2 and 3000 µm2). e Nucleolar area is decreased in sub-sample of C9-ALS neurons compared to control neurons with similar cytoplasmic size. f Nucleolar area in subsamples based on cytoplasmic size. g Distribution of cytoplasmic area in control, C9-ALS and SALS gamma motor neurons. h TDP-43 mislocalization occurs in alpha motor neurons (white dashed outline) and in gamma motor neurons (yellow dashed outline) identified by nuclear ESRRG immunofluorescence (arrowheads)

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