Table 1 Primer sequences for qRT-PCR
From: Non-cell autonomous astrocyte-mediated neuronal toxicity in prion diseases
Primers | Accession number | Sequence |
|---|---|---|
Gfap | NM_001131020.1 | F 5′- ACAGACTTTCTCCAACCTCCAG-3’ R 5′- CCTTCTGACACGGATTTGGT-3’ |
S100b | NM_009115.3 | F 5′- CTGGAGAAGGCCATGGTTGC-3’ R 5′- CTCCAGGAAGTGAGAGAGCT-3’ |
Aldh1l1 | NM_027406.2 | F 5′- CAGTTCTTCAAGGGGTCTGC-3’ R 5′- CAGAATTCGCATCCAAGAGC-3’ |
Glul | NM_008131.4 | F 5′- CCGCTCGCTAAGCTGTTACT-3’ R 5′- CTTTGGTGTTAGAGAGGACAACTTT-3’ |
Itgam | NM_001082960.1 | F 5′- CAGCCTTTGACCTTATGTCATGG-3’ R 5′- CCTGTGCTGTAGTCGCACT-3’ |
Fox3 | NM_001039167.1 | F 5′-ACCTACAGCATCGGAACCAT- 3’ R 5′-TTGCTAGTAGGGGGTGAAGC-3’ |
Mbp | NM_001025251.2 | F 5′- CTCAGAGGACAGTGATGTGTTT-3’ R 5′- CGCCTTGCCAGTTATTCTTTG-3’ |
S100a4 | NM_011311.2 | F 5′- GACTCCTCAGATGAAGTGTTGG-3’ R 5′- GTGCGAAGAAGCCAGAGTAA-3’ |
Vim | NM_011701.4 | F 5′- GTACCGGAGACAGGTGCAGT-3’ R 5′- TTCTCTTCCATCTCACGCATC-3’ |
Lcn2 | NM_008491.1 | F 5′- CCCCATCTCTGCTCACTGTC–3’ R 5′- TTTTTCTGGACCGCATTG-3’ |
Serpina3n | NM_009252.2 | F 5′- GCAACACCCTGGAAGAGATT-3’ R 5′- CTGGGTCTGTTTCCTCACATAG-3’ |
Steap4 | NM_054098.3 | F 5′- CCCGAATCGTGTCTTTCCTATAA-3’ R 5′- CCTCGATAGAGCTGCAGAATG-3’ |
Hspb1 | NM_013560.2 | F 5′- ACAGTGAAGACCAAGGAAGG-3’ R 5′- CTGGAGGGAGCGTGTATTT-3’ |
Cxcl10 | NM_021274.2 | F 5′- AGTAACTGCCGAAGCAAGAA-3’ R 5′- GCACCTCCACATAGCTTACA-3’ |
Timp1 | NM_001044384.1 | F 5′- CCTGGGTTTTCATTGGAGGGTTG-3’ R 5′- GTGGCAGAACTTGAGGAGGTC-3’ |
Serping1 | NM_009776.3 | F 5′- TGATGGCGCCTTTCTTCTAC-3’ R 5′- CCACCTTGGCCTTCAAAGTA-3’ |
H2t23 | NM_010398.3 | F 5′- GATACCTACGGCTGGGAAATG-3’ R 5′- GTGATGTCAGCAGGGTAGAAG-3’ |
Ggta1 | NM_001308300.1 | F 5′- TCATCGGGTCCTACCTACAA-3’ R 5′- CTTCAGTCACCTGCTCCATAC-3’ |
H2D1 | NM_010380.3 | F 5′- CGATGCCTTTGGAGGAGTAT-3’ R 5′- GCATAAGGACGGCAGAATAGA-3’ |
S100a10 | NM_009112.2 | F 5′- GGGCTTCCAGAGCTTTCTATC-3’ R 5′- CTCCAGTTGGCCTACTTCTTC-3’ |
Tgm1 | NM_001161715.1 | F 5′- GCCCTTGAGCTCCTCATTG-3’ R 5′- CCCTTACCCACTGGGATGAT-3’ |
Il66 | NM_031168.2 | F 5′- TGCTGCCACCTTATTTACTCC-3’ R 5′- AGTGGGGCATGATGACAGTT-3’ |
Il12b | NM_001303244.1 | F 5′- GCTTCTTCATCAGGGACATCA-3’ R 5′- CTTGAGGGAGAAGTAGGAATG-3’ |
Il33 | NM_001164724.2 | F 5′- TCCACGGGATTCTAGGAAGA-3’ R 5′- GAGGCAGGAGACTGTGTTAAA-3’ |
Ccl2 | NM_011333.3 | F 5′- TGAGGCTCAGCACAGCAA-3’ R 5′- ATGGGCTTCCAGAATACCG-3’ |
Ccl4 | NM_013652.2 | F 5′- GCCCTCTCTCTCCTCTTGCT-3’ R 5′- GGAGGGTCAGAGCCCATT-3’ |
Ccl5 | NM_013653.3 | F 5′- TGCAGAGGACTCTGAGACAGC-3’ R 5′- GAGTGGTGTCCGAGCCATA-3’ |
Thbs1 | NM_011580.4 | F 5′- CCCCAACCTTCCCAACTC- 3’ R 5′- GGGTTGTAATGGAATGGACAG-3’ |
Thbs4 | NM_011582.3 | F 5′- ATGGGACGGCTGAACAAA-3’ R 5′- GCCATCTCCTCTCTGCAAAT-3’ |
Sparcl1 | NM_010097.4 | F 5′- AAACCATCCCAGTGACAAGG-3’ F 5′- GTCCACCTCGAAGCTGTAGG-3’ |
Gpc4 | NM_008150.2 | F 5′- CCTTGATTTTGAGTGGAACAATTT-3’ R 5′- AATGTTGAAAGGACCCTCCAG-3’ |
Gpc6 | NM_001079844.2 | F 5′- CCTTGATTTTGAGTGGAACAATTT-3’ R 5′- AATGTTGAAAGGACCCTCCAG- 3’ |
Sparc | NM_009242.5 | F 5′- CACCCAGACTCTGTGCTTATT-3’ R 5′- TCAGGTCTCACAGCATCTTTAC-3’ |
Vegfa | NM_001287056.1 | F 5′- CCGCTCGCTAAGCTGTTACT-3’ R 5′- CTTTGGTGTTAGAGAGGACAACTTT-3’ |
Bdnf | NM_007540.4 | F 5′- GCATCTGTTGGGGAGACAAG-3’ R 5′- TCACCTGGTGGAACATTGTG-3’ |
Ntf3 | NM_001164034.1 | F 5′- GGTAATGGAGGGCTTTCTTCTC-3’ R 5′- GCACCGAAGAGAATCAGGTTTA-3’ |
Syp | NM_009305.2 | F 5′- CAAGGCTACGGCCAACAG-3’ R 5′- GTCTTCGTGGGCTTCACTG-3’ |
Syn2 | NM_001111015.1 | F 5′- CCAATCACCGAGAGATGCTTAC-3’ R 5′- CAATGTCCTGGAAGTCGTAGTG-3’ |
Dlg4 | NM_007864.3 | F 5′- CGCTACCAAGATGAAGACACG-3’ R 5′- CAATCACAGGGGGAGAATTG-3’ |
Thbs2 | NM_011581.3 | F 5′- GAACCAACCCTTCGGTGTT-3’ R 5′- TGGATTCTCTGGCTCACACA-3’ |
Gria1 | NM_001113325.2 | F 5′- AGGGATCGACATCCAGAGAG-3’ R 5′- TGCACATTTCCTGTCAAACC-3’ |
Gria4 | NM_019691.4 | F 5′- CTGCCAACAGTTTTGCTGTG-3’ R 5′- AAATGGCAAACACCCCTCTA-3’ |
Tmem119 | NM_146162.3 | F 5′- CCACAGCATTGAGGAGTTTG-3’ R 5′- ACAGCTCATCATTTGGCTCA-3’ |
CD68 | NM_001291058.1 | F 5′- CTGCCAGTCCGAAAATGGAAC-3’ R 5′- CTTCATCCACCGGGGCTATC-3’ |
Tnfa | NM_013693.3 | F 5′- CTGTAGCCCACGTCGTAGC-3’ R 5′- TTGAGATCCATGCCGTTG-3’ |
Il1β | NM_008361.4 | F 5′- AGTTGACGGACCCCAAAAG-3’ R 5′- AGCTGGATGCTCTCATCAGG-3’ |
Il1α | NM_010554.4 | F 5′- CTCTGAGAACCTCTGAAACGTC-3’ R 5′- GAAACTCAGCCGTCTCTTCTT-3’ |
C1qa | NM_007572.2 | F 5′- GAGTCCATACCAGAACCACAC-3’ R 5′- ACAGACAAAGGTCCCACTTG-3’ |
P2ry12 | NM_027571.4 | F 5′- AACACCACCTCAGCCAATAC-3’ R 5′- ACAGCAATGGGAAGAGAACC-3’ |
Trem2 | NM_031254.3 | F 5′- TGGGACCTCTCCACCAGTT-3’ R 5′- GTGGTGTTGAGGGCTTGG-3’ |
Tlr2 | NM_011905.3 | F 5′- CACTATCCGGAGGTTGCATATC-3’ R 5′- GGAAGACCTTGCTGTTCTCTAC-3’ |
Aif1 | NM_001361501.1 | F 5′- GACGTTCAGCTACTCTGACTTT-3’ R 5′- GTTGGCCTCTTGTGTTCTTTG-3’ |
Cx3cr1 | NM_009987.4 | F 5′- GAGAGATGGCTCAGTGGTTAAG-3’ R 5′- CACAGGAACAGGGAGCTATTT-3’ |
Ccl9 | NM_011338.2 | F 5′- CTTGAGCTACAGCCATCCTAAC-3’ R 5′- CAGACCACTCTCACAGTTTATCC-3’ |
Ccl12 | NM_011331.3 | F 5′- GTTCCTGACTCCTCTAGCTTTC-3’ R 5′- GCATCTGGTCCAGCCAATA-3’ |
Ccl6 | NM_009139.3 | F 5′- GGGACAGCATTCTGAACTCTAC-3’ R 5′- CTCACTCCTCCCTGATTCTCT-3’ |
Il10 | NM_010548.2 | F 5′- AGAAAAGAGAGCTCCATCATGC-3’ R 5′- CGGAATTTCTGGGATTCAGCTTC-3’ |
Gapdh | NM_001289726.1 | F 5′- AACAGCAACTCCCACTCTTC-3’ R 5′- CCTGTTGCTGTAGCCGTATT-3’ |