Table 1 Primary Antibodies used in immunofluorescent staining
Antibody | Specificity (antigen/cell type or structure) | Dilution | Host species | Source |
|---|---|---|---|---|
D13 | Prion protein | 1:100 | Human | Ref, Matsunaga |
Cone Arrestin | Arrestin 3/Cone photoreceptors | 1:100 | Rabbit | Millipore, AB15282 |
Cone Opsin | Red, Green opsins/Cone photoreceptors | 1:100 | Rabbit | Chemicon, AB5404 |
Rhodopsin | Rhodopsin/Rod photoreceptors | 1:100 | Rabbit | Millipore, MABN15 |
GNAT1 | G protein subunit alpha transducin 1 /Rod photoreceptors | 1:100 | Rabbit | Abcam, ab74059 |
GNAT2 | G protein subunit alpha transducin 2 /Cone photoreceptors | 1:100 | Rabbit | Thermofisher, PA5-22,340 |
GLUT1 | Glucose transporter 1/Cell membranes of many cell types | 1:100 | Rabbit | Abcam, ab115730 |
CtBP2 | C-terminal binding protein 2/ribeye protein of ribbon synapses | 1:100 | Rabbit, mouse | Invitrogen, PA-79086 Santa Cruz, sc-17759 |
PKCα | Protein kinase C/Rod bipolar cells | 1:100 | Rabbit, mouse | Invitrogen PA5-17,551, MA1-157 |
Calbindin | Calbindin/Horizontal cells | 1:100 | Rabbit | Abcam, ab108404 |
Rootletin | Rootletin/photoreceptor rootlets | 1:50 | Mouse | Millipore, ABN1714 |
Centrin3 | Centrin3/cilia and basal bodies in photoreceptor inner segments | 1:100 | Rabbit | Thermofisher, PA5-35,865 |
Secretagogin (SCGN) | Cone bipolar cellsa | 1:100 | Rabbit | Thermofisher, PA5-30,393 |