Fig. 2

Timecourse of PrP staining with D13 antibody in retina at various times after infection with strain 79A scrapie prions. a Uninfected mouse showing PrPC (magenta) mainly in OPL. b, c At 82 and 104 days post infection (dpi) the misfolded, disease-associated form of PrP (PrPSc) (magenta) can now be seen in the IS and OPL at progressively wider areas. d At 118 dpi, PrPSc is widespread in the IS and deposits are not restricted to discrete individual cells. Some small deposits are visible in the ONL and ONL is beginning to thin as photoreceptors die. e At 131 dpi, PrPSc is deposited in the IS and OPL, and ONL is much thinner. f PrPSc staining appears less at 162 dpi in IS and OPL, and ONL is dramatically thinner. Punctate PrPSc is present in IPL (arrowhead). Scale bar in a = 20 µm