Fig. 10

Changes in rod bipolar cells during infection. a anti-PKCα staining (green) in an uninfected mouse shows normal distribution and morphology of rod bipolar cells. Numerous dendrites (arrow) reach upward to synapse with photoreceptors in ONL from each cell body (arrowhead). b At 104 dpi rod bipolar cells appear similar to uninfected mouse. c By 118 dpi the number of bipolar cells has dropped to about half vs uninfected and some cell bodies have few if any associated dendrites (arrowhead). d Late in disease, at 163 dpi, rod bipolar cell number is significantly reduced compared to uninfected retina, and few dendrites reach into OPL (arrow). e The number of rod bipolar cells per 100 µm of retina over time. Each dot represents counts from one field of view, line is median, ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p 0.0001 statistics by one-way ANOVA with multiple comparisons test. Scale bar in a = 20 µm