Fig. 1

Reduction of Nlgn1 band in parietal cortex of AD brain. a Detection of Nlgn1 with antibodies targeting different epitopes by immunoblot. TBS soluble fractions of 4 Ctrl and 4 AD brain samples, 20 µg protein/lane. Samples 3–8–17–2–10–11-14 are from superior parietal cortex, while sample 7 is from inferior parietal cortex. a The blue-boxed areas indicate the common Nlgn1 immunoreactive band at around 80 kDa. Recombinant Nlgn1 protein, corresponding to the extracellular part fused to a C-terminal 6-His tag, is shown in the first lane. b Significant reduction of Nlgn1 band intensity, detected by a-Nlgn1 antibody from Synaptic System. c Significant reduction of Nlgn1 band intensity, detected by a-Nlgn1 (F-7) antibody. The bars presented in the scatterplots show the median and the interquartile range. Significance: p  ≤ 0.05. d–e Western blots showing the specificity of F-7 and SySy a-Nlgn1 antibodies toward Nlgn1 recombinant protein. Ab = antibody