Fig. 4
UbG76V-GFP reporter does not accumulate in the striatal projections of the SNpc following 1 week of A53T α-synuclein over-expression. Adult wild-type rats received unilateral stereotaxic co-injection of AAV-UbG76V-GFP with AAV-A53T, AAV-Empty or AAV-TdTomato into the right SNpc. Animals were culled at 1 wpi. Representative immunofluorescent staining of ipsilateral striatum with anti-tyrosine hydroxylase (TH; cyan), anti-GFP (GFP; green), anti-α-synuclein (SYN; red) antibodies or native TdTomato fluorescence (TdTom; red) at 1 wpi is shown (n = 3 per group). No evidence of UbG76V-GFP reporter accumulation in striatal terminals is observed, despite widespread expression of A53T and TdTomato transgenes. Scale bar 500 μm