Skip to main content
Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Early-onset impairment of the ubiquitin-proteasome system in dopaminergic neurons caused by α-synuclein

Fig. 2

Co-expression of AAV-UbG76V-GFP viral UPS reporter does not modify A53T α-synuclein expression, dopaminergic neuronal loss or forelimb asymmetry in AAV-A53T treated rats. Adult wild-type rats received unilateral stereotaxic injection of AAV-A53T or AAV-Empty, either alone or in combination with AAV-UbG76V-GFP, into the right SNpc. Animals were culled 3 weeks following virus administration. a Representative images of anti-tyrosine hydroxylase (TH; cyan) and anti-α-synuclein (SYN; red) immunofluorescent staining of coronal cryosections show similar patterns of A53T α-synuclein expression and dopaminergic neuronal loss in ipsilateral SNpc of animals injected with AAV-A53T and AAV-A53T/AAV-UbG76V-GFP. Scale bar 500 μm. b Quantification of TH-labelled cells in SNpc ipsilateral to site of AAV injection. A significant reduction in the density of dopaminergic neurons is observed at 3 wpi with no significant difference (n.s.) between AAV-A53T and AAV-A53T/AAV-UbG76V-GFP-treated groups. Data are mean +/− SEM (*p < 0.05; one-way ANOVA with Tukey post-hoc tests; n = 6–8 per group). c Measurement of forelimb asymmetry in the cylinder test at 3 wpi. Rats injected with AAV-A53T display significant asymmetry in forelimb use with no effect of co-administration of AAV-UbG76V-GFP. Data are percentage mean +/−SEM (*p < 0.05; **p < 0.01; ***p < 0.001; one-way ANOVA with Tukey post-hoc tests; n = 7–15 per group)

Back to article page