Fig. 4

Time-lapse image of microtubule polymerization. a SH-SY5Y cells were transfected with EB3-GFP fusion vector, and laser scanning live confocal images were taken 24 h after DNA transfection at 0.53 fps for 40 frames with Em/Ex 488/520 nm using 100× Oil objective (NA = 1.45). Arrowheads indicate one of the EB3-GFP comets polymerizing outward from the microtubules. Scale bar: 5 μm. b Velocities of EB3 comets were quantified after co-transfection of EB3-EGFP (0.2 μg) with/out HA-TTBK1 (0.4 μg), followed by treatment with 10 μM freshly dissolved Aβ42 at 1 h prior to the imaging. The error bars indicate SEM. *** denotes p < 0.001 by one-way ANOVA with Turkey post hoc (N = 10 cells per group). ANOVA F (2, 688) = 22.36 (p < 0.0001)