Fig. 1From: Tau-tubulin kinase 1 and amyloid-β peptide induce phosphorylation of collapsin response mediator protein-2 and enhance neurite degeneration in Alzheimer disease mouse modelsLocalization of TTBK1 and pCRMP2 in the pyramidal neurons of the EC and CA1–3 hippocampal regions in early AD brains. The human brain specimens are listed in Table 1. a Deparaffinized human brain sections (8 μm in thickness) containing the EC layer II and CA1 hippocampal regions from patients in Braak I-IV were DAB immunostained for TTBK1 and counterstained with hematoxylin. Top panel: EC regions, bottom panel: CA1 region. Scale bar = 20 μm. b Immunofluorescence of Braak II EC brain sections with AT8 (pSer202/205 tau, green), TTBK1 (red) and Dapi (blue). Scale bar = 10 μm. c DAB immunostaining with 3F4 (pCRMP2) in the EC layer II and CA1 hippocampal regions from patients in Braak I-IV. Scale bar = 20 μm. d Co-localization of 3F4 (pCRMP2) and pSer422 Tau in the CA1 region of Braak I brain sections (indicated by *). Scale bar = 40 μmBack to article page