Table 2 Antibody details
Target | Marker | Host | Clonality (clone) | End concentration | Company (catalog number) | Antigen retrieval |
|---|---|---|---|---|---|---|
APCs | MHCII | Mouse | Monoclonal (LN3) | 6.8 μg/mL | Hybridoma | Citrate |
B cells | CD19 | Rat | Monoclonal (6OMP31) | 0.5 μg/mL | Thermo Fisher Scientific (14–0194-82) | Citrate |
Basement membrane | Collagen IV | Rabbit | Polyclonal | 3.3 μg/mL | Abcam (ab6586) | Tris or citrate |
CD4+ T cells | CD4 | Rabbit | Monoclonal (EPR6855) | 1.1 μg/mL | Abcam (ab133616) | Tris |
CD8+ T cells | CD8 | Mouse | Monoclonal (C8/144B) | 0.157 μg/mL | Dako (M7103) | Tris |
Endothelial cells | Biotinylated UEA I Europaeus Agglutinin I (UEA I) | 2.0 μg/mL | Vector laboratories (B-1065) | Tris | ||
Granulocytes | CD66b (A647 label) | Mouse | Monoclonal (G10F5) | 4.5 μg/mL | Novus Biologicals (NB100-77808AF647) | Citrate |
Myeloid cells | Iba1 | Goat | Polyclonal | 1.0 μg/mL | Abcam (ab5076) | Citrate |
Plasma cells | CD138 | Mouse | Monoclonal (MI15) | Not available (1:50) | Thermo Fisher Scientific (MA5–12400) | Citrate |
T cells | CD3 | Mouse | Monoclonal (F7.2.38) | 2.8 μg/mL | Dako (M7254) | Tris |
T cells | CD3 | Rabbit | Polyclonal | 3.0 μg/mL | Dako (A0452) | Tris |