Fig. 1

Density of macrophages and DCs is similar in the CP of progressive MS patients and controls. a Example of the compartmentalization of the CP tissue. Using the basement membrane as guidance (collagen IV, in white), we discern the stromal and epithelium-associated cells (which together are the CP cells), and exclude the circulating cells located in the vessels. b Maximum projection of an image of a CP immunolabeled with Iba1, MHCII and collagen IV. Right panel shows a higher magnification of the image. White arrowheads point to one MHCII+ and one MHCII- macrophage (Iba1+) in the CP stroma; white arrow points to a dendritic cell (DC; Iba1- MHCII+) associated to the epithelium. c Density of CP macrophages (MHCII+ and MHCII-) in control and progressive MS (Wilcoxon rank sum test with continuity correction). d Density of CP macrophages in the different CP compartments (Welch Two Sample t-test and Wilcoxon rank sum test with continuity correction). e Density of CP DCs in control and progressive MS (Wilcoxon rank sum test). f Density of CP DCs in the different CP compartments (Wilcoxon rank sum test and Welch Two Sample t-test). Col IV: collagen IV; PMS: progressive MS. Scale bars are 100 μm