Fig. 9From: Pre-clinical characterisation of E2814, a high-affinity antibody targeting the microtubule-binding repeat domain of tau for passive immunotherapy in Alzheimer’s diseaseSize exclusion chromatography of tauopathy brain samples. Human brain homogenates from AD (upper panels) or PSP (lower panels) patients were loaded onto a Superdex 200 Increase GL size exclusion column in PBS and 1 mL fractions were collected. Samples from each fraction were resolved by SDS-PAGE and tau proteins were detected by western blotting using antibodies raised against two different parts of the full length protein: HT7 (0.2 μg/mL) or 7G6 (0.5 μg/mL) as indicated in the left or right panels, respectively. Images shown are representative from four different AD and six PSP brainsBack to article page