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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: Pre-clinical characterisation of E2814, a high-affinity antibody targeting the microtubule-binding repeat domain of tau for passive immunotherapy in Alzheimer’s disease

Fig. 2

Fine epitope mapping of 7G6 and E2814 anti-tau antibodies. Overlapping peptides covering the full length wild type human 2N4R tau sequence were synthesised and printed onto glass chips. Each chip was then probed with either a) 7G6 (mouse) or b) E2814 (human) antibodies at 1 μg/mL. Bound antibodies were detected by the addition of fluorescently-labelled secondary antibodies and images captured using a LI-COR Odyssey machine. Fluorescent spots corresponding to antibody-bound peptides were quantified and intensity plots spanning the full tau sequence were generated for both murine 7G6 (a) and human E2814 (b) antibodies. Both antibodies bound peptides containing an HVPGG sequence as indicated in each panel

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