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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Wwox deficiency leads to neurodevelopmental and degenerative neuropathies and glycogen synthase kinase 3β-mediated epileptic seizure activity in mice

Fig. 5

Foliation defect, Purkinje cell loss and neuronal apoptosis in the cerebellum of Wwox−/− mice. a Representative images of Wwox+/+ and Wwox−/− brains revealed that interhemispheric fissure (arrowheads) and cerebellar vermian lobules VI and VII (arrow) were fused in Wwox−/− brain. b Midline sagittal sections of Wwox+/+ and Wwox−/− cerebellum tissues were stained with cresyl violet. A smaller lobule V and fusion of VI with VII were observed in Wwox−/− mouse cerebellum. Scale bars = 500 μm. c H&E staining of the cerebellar cortex tissue sections from Wwox−/− mice (N = 6) showed partial loss of Purkinje cells (arrows) at postnatal day 20. The lower panel pictures are the magnified images from the boxed area in the upper panel. Scale bars = 200 (upper) or 50 μm (lower). d The numbers of Purkinje cells in ten representative subareas of Wwox+/+ and Wwox−/− cerebellar cortex tissue sections were quantified. The results are expressed as means ± SEM. **P < 0.01. e Apoptotic cell death was detected in the granular cells of Wwox−/− cerebellum at postnatal day 20 by TUNEL assay. The representative results of four independent experiments are shown. PC, Purkinje cell; GL, Granule cell layer; ML, molecular layer. Scale bars = 200 μm

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