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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Wwox deficiency leads to neurodevelopmental and degenerative neuropathies and glycogen synthase kinase 3β-mediated epileptic seizure activity in mice

Fig. 3

Peripheral nerve degeneration and Schwann cell apoptosis in Wwox−/− mice. a Semi-thin toluidine blue-stained transverse sections of sciatic nerves embedded in EMbed 812 resin from Wwox+/+ and Wwox−/− mice at postnatal day 20 are shown (N = 3). Scale bars = 50 μm. b Electron microscopy revealed normal ultrastructural features of axons (Ax), myelin sheath (My) and endoneurium (En) in EMbed 812 resin-embedded sciatic nerve sections from Wwox+/+ mice. In contrast, abnormal-shaped nerve fibers (red stars), axon demyelination (blue arrow) and onion bulb degeneration (red arrowheads) were observed in all Wwox−/− sciatic nerves examined in this study (N = 3). Red arrows indicate detachment of myelin lamellae with invasion towards the axolemma due to loss of axoplasm in Wwox−/− axons. A significant reduction in endoneurium spaces was evident in Wwox−/− sciatic nerves. Scale bars = 5 μm. c By immunogold-labelling, cleaved caspase-3 was detected in the Schwann cell (Sc) from Wwox−/− sciatic nerve, photographed at 50,000X magnification. The representative images of three independent experiments are shown

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