Skip to main content
Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: A robust TDP-43 knock-in mouse model of ALS

Fig. 6

Mis-regulation of Bcl-2 expression and calcium ion homeostasis in cultured (N390D/+) MN. a Comparison of the levels of TDP-43 at early stages of ESC-derived MN in culture (from day 1 to day 3), as analyzed by Western blotting. The statistical analysis is shown in the lower scatter plot (mean ± SD). b Comparison of the expression levels of Bcl-2-201mRNA and Bcl-2 protein as analyzed by RT-PCR (exemplified in the upper panels) and Western blotting (exemplified in the middle panels), respectively. The statistical analysis is shown in the lower scatter plots (mean ± SD). c Comparison of the levels of Bcl-2 mRNA by RT-PCR (upper panel) and Bcl-2 protein by Western blotting (lower panel) and in cultured (+/+) MN and (N390D/+) MN on day 2 and day 14, respectively. d RT-PCR detection and statistical analysis (left scatter plot, mean ± SD) of the changes of the relative ratio of the two Bcl-2 mRNA isoforms. Note the increase of functional Bcl-2 -201 mRNA in the spinal cord of N390D/+ male mice at both pre-symptomatic (3-month) and symptomatic (6-month) stages. N = 3 (randomly chosen from each of the two independent lines) per group. e The relative levels of the cytosolic calcium ion levels in cultured MN (left plot) and transfected HEK-293 T cells (right plot) overexpressing human TDP-43(wild-type; WT), TDP-43 (A315T) or TDP-43 (N390D). The relative calcium ion levels were analyzed with the use of Fura2-AM reagent and compared in the separated scatter plots (mean ± SD). Note the overloading of the cytosolic calcium ion in cultured (N390D/+) MN and in HEK-293 T cells overexpressing TDP-43 (N390D) in comparison to TDP-43 (WT) and TDP-43 (A315T). Significantly different represented in a-e: * p < 0.05, ** p < 0.01, # p < 0.001. n.s., not significant

Back to article page