Behavioral analysis of TDP-43 PFFs M1-C and PBS M1-C mice. Rotarod test analysis (a) and hanging wire test (b) of mice at various time post injection. Footprint analysis of the left forelimb stride length (c), right forelimb stride length (d), left hindlimb stride length (e), right hindlimb stride length (f), fore-base width (g), hind-base width (h) at various time post injection. H&E staining for the right biceps brachialis muscles of TDP-43 PFFs M1-C mice (i) and PBS M1-C mice (j). Quantity of muscle fibers at different cross-sectional areas (k). Morris water maze analysis of escape latency (l), path length (m), swim speed (n), probe trial (o) at various time post injection. n = 9 mice/group. The error bar in all panels represents the Standard Error of Mean (SEM). Data are the mean ± SEM. Statistical analysis was performed using the Student’s t test.