Heat maps of TDP-43 pathology in coronal sections stained with pTDP-43 antibody. Semi-quantitative analyses of the burden of TDP-43 pathology in neurons and in white matter tracts in the brain and spinal cord of TDP-43 M1-C mice at different times (n = 3 at 1 mpi, n = 3 at 2 mpi, n = 3 at 6 mpi, n = 3 at 12 mpi) after TDP-43 PFFs injection. Each panel represents heat map pathology distribution on one of the six different coronal planes (Bregma 0.74 mm, − 1.70 mm, − 3.80 mm, − 7.64 mm, C7 section, L3 section) for the different post-injection times. The mean values of the TDP-43 pathology were graded from negative (0, gray) to most abundant (3, red) and color-coded onto heat maps. The panels in the far left column shows sagittal views of the corresponding coronal planes (blue line) and the site of injection into cortex was indicated by the red star.