Fig. 6
Transcriptomic analysis of hippocampus from R514G mice. PCA (a) and volcano (b) diagnostic plots for transcriptomic analysis of 12-month-old R514G-FUS mice, WT-FUS and non-transgenic controls showed changes in gene-expression. Red, blue and grey points represent upregulated genes, downregulated genes and genes below our significance threshold, respectively. c Heatmap of Z-centered log-scaled counts of differentially expressed genes across all samples. d The top overrepresented GO (Biological Process) terms in the up and down regulated differentially expressed genes in R51G mice as compared to non-transgenic mice. e The top enriched and depleted Reactome pathways resulting from gene set enrichment analysis. The height and color gradient of the bars reflect the normalized enrichment score and the log(p-value) of the Reactome pathway gene-sets in R514G-FUS mice, while the height of the points reflect the log2(foldchange) of the genes constituting the gene set in R514G-FUS mice. Majority of the genes in each gene-set are dysregulated in the same direction of the overall gene-set enrichment