Fig. 5

R514G-FUS animals show hippocampus-dependent cognitive deficits. a A schematic showing the spatial acquisition of the hidden platform in the Morris water maze paradigm. b, c The latencies to reach the hidden platform across five training days at 6 and 12 months. The latencies of R514G-FUS animals remained high and were significantly higher than non-transgenic animals on training days 4 and 5 (ps < 0.05). d, e Two probe tests were performed with the short-term memory probe carried out 20 min after the first reversal training session, and the long-term memory probe carried out 16–24 h after the fifth training session. R514G-FUS mice spent less time in the target quadrants during the STM and LTM probes compared to non-transgenic mice. f, g The average graphical representation for the search pattern of R514G-FUS and non-transgenic mice during each probe that was carried out at 6 and 12 months. h A schematic diagram showing the contextual fear conditioning paradigm that was used to assess hippocampal-dependent contextually acquired fear memory. i, j Percentages of freezing during training were recorded during the contextual fear conditioning paradigm. There were no significant differences in the percentages of time spent freezing between R514G-FUS and non-transgenic mice across both ages. k, l Percentages of freezing during probes were recorded during the contextual fear conditioning paradigm. R514G-FUS mice showed reduced freezing during the long-term and remote memory probes at both 6 and 12 months old. For all the tests shown here, there were 5 R514G-FUS mice and 6 non-transgenic mice. Orange circles and bars represent R514G-FUS mice, and black circles and grey bars represent non-transgenic mice