Fig. 3

Reduction of long-term potentiation and spine density in the hippocampus of R514G mice. a, b LTP measurement of 6- and 12-month-old non-transgenic littermate control and R514G-FUS mice. fEPSPs were recorded in stratum radiatum (dendritic area) of the CA1 region following the stimulation of the Schaffer collateral pathway. LTP were induced with 4 tetanus stimulation delivered at 20-s intervals, each at 100 Hz for 1 s. Both 6- (a) and 12 months old (b) R514G-FUS mice showed reduced LTP when compared with their littermate controls. 6-month time-point: non-transgenic mice, n = 6; R514G-FUS mice n = 5. 12-month time-point: non-transgenic mice, n = 9; R514G-FUS mice n = 9. c Representative dendrite images of 6 month and 12 month old non-transgenic littermate control and R514G-FUS mice. Scale bar is 10 μm. d Quantification of spine density in 6 months and 12 months old non-transgenic littermate control and R514G-FUS mice. Spine density was reduced in the 6 and 12 months old R514G-FUS mice. Data are expressed as mean ± SEM; asterisks indicate significant differences between groups, n = 3 per genotype per time-point (*p < 0.05; unpaired t-test)