Fig. 5

14-3-3θ overexpression mitigates reduction in nigral TH-positive neuronal counts in response to PFFs, while 14-3-3 inhibition exacerbates reductions in dopaminergic neurons. a Representative images of TH immunohistochemistry in the SN of AAV-GFP and AAV-14-3-3θ/GFP mice injected with αsyn monomers or PFFs at 6 mpi. Scale bar = 500 μm. b Stereological counts of TH-positive neurons in the SN of AAV-GFP and AAV-14-3-3θ/GFP mice injected with αsyn monomers or PFFs at 6 mpi. n = 9–14 per group. *p < 0.05 (Tukey’s multiple comparison test). n.s. = non-significant. Error bars represent SEM. c Representative images of TH immunohistochemistry in the SN of WT and difopein nigral mice (line 166) injected with αsyn monomers or PFFs at 6 mpi. Scale bar = 500 μm. d Stereological counts of TH-positive neurons in the SN of WT and difopein mice injected with αsyn monomers or PFFs at 3 mpi. n = 7–8 per group at 3 mpi. Error bars represent SEM. e Stereological counts of TH-positive neurons in the SN of WT and difopein mice injected with αsyn monomers or PFFs at 6 mpi. n = 11–14 at 6 mpi. *p < 0.05, **p < 0.01, ****p < 0.0001 (Tukey’s multiple comparison test). Error bars represent SEM